Cover Image

EQUIPMENT    Microscope  Preventative  Maintenance  
Preventative  Maintenance    Complete  every  six  months.  

• Clean  machine  with  air  brush  or  air  blower,  lint  free  cloth  with  lens  cleaner.  Start  cleaning  objective  lens  from  center  and  spiral  out  with  cotton  swab  or  cloth.  If  lens  cleaner  is  not  available,  ethyl  ether,  xylene,  petrol  can  be  used.  However,  alcohol,  acetones  or  any  other  ketones  should  not  be  used,  as  they  may  dissolve  the  sealants  around  the  lens.  • Check  for  and  remove  any  present  fungal  growth.  • Ensure  a  specimen  can  be  viewed  clearly  through  all  objectives.  Do  not  scratch  or  damage  the  lens.  Oil  lens  -­‐  X100  needs  a  drop  of  oil  to  confirm  clear  image.  Clean  all  objectives  and  eyepieces  first,  and  then  check  for  image  clarity.    • Check  optical  alignment.  • Lubricate  adjustments.  • Ensure  light  source  is  working  properly.  • Inspect  for  signs  of  damage,  scratches,  or  dirt.  • Examine  switches  and  controls  for  proper  function.  • Replace  uncooked  rice  before  storage  to  prevent  fungal  growth.    Kohler  Illumination:    It  is  a  method  of  illumination  which  involves  optimizing  a  microscope’s  optical  train  to  produce  homogenously  bright  light  free  from  artifacts  and  glare.  In  Kohler  illumination,  four  separate  planes  combine   to   form   conjugate   planes   in   both   the   illumination   and   image-­‐forming   light   pathways.   The   lamp  filament,  aperture  diaphragm,  back  focal  plane  of  the  objective  lens,  and  the  eye  point  which  is  approximately  one  centimeter  above  the  top  lens  of  the  ocular,  form  the  illumination  conjugate  plane.  The  conjugate  planes  of  the  imaging  light  path  are  the  field  diaphragm,  specimen,  the  fixed  diaphragm  of  the  ocular,  and  the  retinal  plane   of   the   viewer.   In   Kohler   illumination   the   collector   lens   or   field   diaphragm   collects   light   from   the  illumination  source  and   focuses   it  at   the   front   focal  plane  of   the  sub-­‐stage  condenser’s  aperture  diaphragm  which,  in  essence,  projects  an  image  of  the  lamp  filament  onto  the  lens.  

Copyright © 2017 Engineering World Health. All Rights Reserved.